骨头/牙齿
推荐样品量：>1g
样品前处理：骨胶原提取
测试周期：2-3周
骨胶原是骨头样品最好的测年部分，保存完好的骨头呈褐色或棕色，光滑且略带光泽，表面比较坚硬，通常这
种样品能提取到较好的骨胶原蛋白。保存较差的骨头会呈白色或粉末状，矿物质积累也可以被识别，如锰和铁
元素，表明胶原蛋白流失。
Bone/tooth Samples Radiocarbon Analysis methods:
The preparation of bone/tooth samples involves standard acid-base-acid (ABA)procedure and collagen extraction. Firstly, bone/tooth
samples are ultrasonicated in ultrapure water, dried, grinded and sieved to get the appropriately sized sample fraction (0.5–1 mm).
Then samples are treated with 0.5M hydrochloric acid (~18 hrs), 0.1-0.2M sodium hydroxide (30 min-1h), and 0.5M hydrochloric
acid (1 hr). Bone/tooth collagen gelatinization is performed in pH 3 solution at 70°C for 20 hrs. Gelatine solution is filtered using a
cleaned filter and freeze-dried. The quality of collagen is monitored by carbon and nitrogen content in collagen, atomic C/N ratio
determination and collagen yield. Samples are dated if the collagen yield is above 1% and the C:N ratio of the collagen is between
2.9 and 3.5. The samples that deviate from these ratios are deemed unsuitable for dating(van Klinken, G.J.,1999). The pure collagen
samples were combusted with CuO and silver in vacuo to CO2 at 900ºC for 2 hrs. The CO2 was purified, graphitized and measured
on a compact 0.5 MeV NEC Accelerator Mass Spectrometry in the Guangzhou Institute of Geochemistry, Chinese Academy of
Sciences (Zhu et al., 2015).
1. van Klinken, G.J. (1999). Bone Collagen Quality Indicators for Palaeodietary and Radiocarbon Measurements, Journal of archaeological science, 26(6),
pp. 687–695. Available at: https://doi.org/10.1006/jasc.1998.0385.
2. Zhu, S. Y., Ding, P., Wang, N., Shen, C. D., Jia, G. D., & Zhang, G. (2015). The compact AMS facility at Guangzhou Institute of Geochemistry, Chinese
Academy of Sciences. Nuclear Instruments & Methods in Physics Research Section B-Beam Interactions with Materials and Atoms, 361, 72-75.
https://doi.org/10.1016/j.nimb.2015.06.040
